Why you should select Radiance as a tool for your success blot? Shelf life is also longer than Super Signal West Femto. Using Radiance Plus, you can minimize the working time compared to Clarity Max Western ECL, only 2 minutes are enough for protein detection. Furthermore, the signal duration is lasted for up to 8 hours after incubation, higher than Thermo Scientific Pierce ECL Plus. The quantification of the protein could be done accurately. With its high sensitivity and signal-to-noise ratio, you can measure the very low-abundance protein without background. Radiance Plus provides the best ability to detect and quantify an extremely low-abundance protein, in t he low femtograms range. Comparable efficacy compared to SuperSignal™ West Dura at a cheaper price!įigure B: Radiance Q provides an extended dynamic linear range, the linear relationship between the amount of protein and signal density is wider which enhances the accuracy of protein quantification. Moreover, with its long-lasting signal, you can re-expose several times up to 10-24 hours after incubation with only 30% signal strength decay per 1 hour. It is validated to be more sensitive and extended efficiency to determine the amount of protein, accurately with a wider linear range. Radiance Q provides a better ability to detect low-abundance protein, down to mid femtogram range, more sensitive than Thermo Pierce ECL Plus and Amersham ECL Prime. In addition, the signal can be long lasting to 6-8 hours and it is stable at room temperature for up to 1 year, which is longer, compared to Thermo Pierce ECL, Amersham ECL, Biorad Clarity and Supersignal West Pico Plus. Radiance ECL provides high sensitivity for protein detection in high femtogram range. Radiance, the high standard horseradish peroxidase (HRP) substrates to improve protein detection and quantification. Radiance from Azure Biosystem: a novel potential tool for chemiluminescent detectionĪzure Biosystem is delighted to present the new series of ECL for Western Blot analysis. > Therefore, choosing the right ECL substrate that can facilitate protein detection in Western Blot, improving better results. A good ECL should minimize the amount of primary antibodies and the time you need for the processes. To make your blot succeed, the chosen enhanced chemiluminescent (ECL) should provide high sensitivity, less background, and a great linear range for quantification. However, the benefits also come with some drawbacks including a low signal-to-noise ratio, which generates high background and interferes with accurate protein quantification. Chemiluminescent Western Blot has become a widely used technique as it can detect a single protein with high sensitivity, in a low amount range. In Western Blot analysis, a way to detect protein is an important part of the process. Why does choosing the right ECL matter for Western Blot?
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